Marco Uccellini (1603-1680) was born into a noble family in Fulinpopoli, northeastern Italy. The family owned land in the area since the early 14th century and many members held religious positions there, including Marco Uccellini's father Pietro Maria. Uchelini was likely to have studied at the seminary in Assisi in the early 1630s.

According to his will, he may have started his formal music education under the leadership of another famous early violin composer, Giovanni Battista Buonamente, who was the music director of the Saint Francis Cathedral in Assisi at the time.

From 1641 to 1662, Uchelini served at the court of Este in Modena. From 1647 to 1665, he became the principal musician of Modena Cathedral.

Based on salary records and some retained letters from Uchelini, it is evident that he was a highly regarded advisor to the Este family, receiving a salary almost eight times that of other court violinists.

After Duke Alfonso IV of Este joined the Estesen Church, Uchelini lost his position. However, with the help of Isabella d'Este, who had just married into the Farnese family, he quickly became a musician at the Farnese court in Parma in 1665 until his death.

In the Farnese court, he created operas and ballets, but his music did not survive. Therefore, he is known today for his instrumental music alone.

Ucherini was one of the outstanding Italian violin composers of the first half of the 17th century. His violin sonatas and digital bass sonatas have contributed to the development of the conventional writing style of the violin, including flashy transitional phrases, jumps and thrusts into high positions, expanding the range of instrument techniques and expressiveness.

At that time, composers usually did not specify the instruments in their works, but only wrote parts suitable for instruments in similar vocal ranges. Uchelini was one of the early creators of solo violin and digital bass music, and was considered to be of great significance for the rise of secular and instrumental music, as well as the development of violin techniques.

Uchelini's innovation influenced a generation of violin composers, including Johann Heinrich Schmelzer, Heinrich von Biber, and Johann Jakob Walther from the regions of Austria, Germany, Italy, and Bohemia under the rule of the Holy Roman Empire. Famous Baroque masters such as Antonio Vivaldi, Arcangelo Corelli, and Giuseppe Tartini were also his successors in a broad sense.